Electrophoretic Mobility Shift Assay (EMSA) for Detecting Protein-Nucleic Acid Interactions
Electrophoresis was carried out at room temperature in a 5% w/v polyacrylamide gel run in 45 mM Tris-borate (pH 8.0), 2 mM EDTA, 20 μM cAMP. Symbols: F, free DNA; C 1 , C 2 and C 3 , complexes with 1, 2 and 3 CAP dimers bound per DNA molecule,
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(PDF) A Guide to Polyacrylamide Gel Electrophoresis and Detection
For protein separation, virtually In gel electrophoresis, proteins do not all enter the all methods use polyacrylamide as an anticonvective, gel matrix at the same time. Samples are loaded sieving matrix covering a protein size range of into
Zwitter-ion polyacrylamide
Zwitter-ion polyacrylamide is a type of water-soluble macromolecular polyelectrolyte.Its molecular chain has cationic groups and anionic groups simultaneously and can lead to charge neutralization with the active adsorption groups in sludge or
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Anionic polyacrylamide 9003-05-8,1_OKCHEM Advantage Description Anionic polyacrylamide is a type of water-soluble high-molecular polymer and has such properties as flocculability, thickening, shear, resistance diminishing, and dispersion.
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